Ion Exchange Chromatography Solutions for Every Application
Ion exchange chromatography separates compounds based on net surface charge. Molecules are classified as either anions (having a negative charge) or cations (having a positive charge). Some molecules (e.g., proteins) may have both an anionic and cationic group. A positively-charged support (anion exchanger) will bind a compound with an overall negative charge. Conversely, a negatively-charged support (cation exchanger) will bind a compound with an overall positive charge.
Ion exchange matrices can be further categorized as either strong or weak. Strong ion exchange matrices are charged (ionized) across a wide range of pH levels. Weak ion exchange matrices are ionized within a narrower pH range.
Pall offers ion exchange resins and membranes. In many areas, chromatography resins are the media of choice for chromatography applications, but in some cases where resin-based methods have limitations (e.g., purification of viruses or large molecules) membranes have proven to be a robust, scalable, and economic alternative. Membranes perform well in such applications because of their faster flow rates compared to resins.