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TOTAL RNA PURIFICATION

Transcription of genes into RNA is an important step in the synthesis of functional gene products

Advancing sample preparation

Providing smooth flow and rapid processing

High throughput gene expression analysis is used in current genetic research to provide insights into normal, abnormal or pathological cellular processes. Transcription of genes into RNA is an important step in the synthesis of functional gene products, which can be functional RNA species themselves or protein products formed after translation of messenger RNAs. 

 

In many high throughput gene expression analysis studies, cultured mammalian cells are exposed to different reagents or growth conditions, and reverse-transcription quantitative PCR (RT-qPCR) approaches are used to measure the influence of the various conditions on the expression of genes of interest. These types of studies are greatly aided by the availability of multi-well filter plates with silica based media that allow high throughput total RNA isolation from cultured mammalian cells in a robust fashion. 

 

The Pall AcroPrep Advance 96-well Long Tip Filter Plate for Nucleic Acid Binding incorporates a silica-based quartz glass fiber media to allow for efficient binding of DNA and RNA, while providing smooth flow and rapid processing of samples. This media offers researchers the flexibility to purify plasmid DNA from bacteria, and genomic DNA or total RNA from cell culture samples: a single plate for multiple applications. Reducing the chance of cross contamination is critical for reproducible quality results. Pall’s new long tip plate minimizes hanging drop formation thus reducing the possibility of cross contamination. The Pall AcroPrep Advance nucleic acid binding plate is a multipurpose plate providing flexibility in applications, reduced risk of cross contamination and smooth flow for sample processing.

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Discover the benefits of the AcroPrep™ Advance 96-Well Filter Plates for yourself – choose your application and request a sample.