You are running an unsupported browser, please upgrade your browser. Close
Laboratory

Protein Sample Prep & Detection

We look forward to addressing your processing challenges with accuracy, speed, safety and reliability.

Prepped for success

Advanced filtration, analysis and purification solutions for protein preparation and detection.

Reliable sample preparation and fractionation processes are key to the success of proteomic research. Effective steps can facilitate the discovery of life-saving and life-enhancing diagnostic and pharmaceutical products. Ineffective steps can lead to loss of valuable samples and time. We manufacture membranes and chromatography resins that exhibit high resolution and binding capacities with low non-specific binding for the purification and concentration of proteins. These media are available incorporated into devices that use fast, gentle methods and minimise handling to protect samples.

 

As samples get smaller and more numerous, the need for novel methods to purify proteins and improve assays has led Pall to develop a broad line of multi-well filter plates that target specific application challenges. AcroPrep Advance filter plates are designed to provide superior performance in a multitude of sample prep and screening applications. These multi-well filter plates have been optimized to support the stringent requirements of high throughput applications.

 

 

The sealing mechanism used in the AcroPrep Advance filter plate individually seals each well to help prevent cross-talk or cross contamination of samples. The advanced fluid director on the bottom of the plate also minimizes cross contamination of the filtrate during processing.

 

Our ultrafiltration centrifugal devices simplify many common protein handling procedures. These devices provide efficient concentration and salt removal of samples from 50 μL to 60 mL in just minutes. Choose from membranes that have been developed to assure low nonspecific biomolecule binding and provide consistent, high recovery of target molecules. Ultrafiltration reduces the amount of handling that can cause damage to samples, leaving concentrated samples ready for direct incorporation into downstream applications at critical stages in the discovery process.

 

 

We also offer chromatography products to facilitate research needs, scale-up, and polishing. Our chromatography solutions are available in resin or membrane formats to support your specific application. Choose from our extensive portfolio of media including flat sheet membrane, bulk resin, and media incorporated into specific product housings.

 

Our Mustang® membranes are recommended in chromatography applications when there is a need to purify large molecules or in situations where faster flow is required. Membrane chromatography is extremely economical because flow rates are significantly faster than traditional resin chromatography, decreasing processing time and increasing throughput. Pall’s Mustang membranes possess large convective pores and have dynamic binding capacities that are relatively insensitive to the effects of high flow rates, even for large molecules such as plasmids and viruses.

 

For protein characterization, the use of membrane technology is ideal for obtaining detailed structural information. Pall offers an impressive range of membranes compatible with all common protein detection procedures. Our membranes feature superior binding capacities and extremely low background to facilitate the transfer and retention of rare and low-abundant proteins for detection and further characterization.

 

 

Pall’s strict quality manufacturing specifications ensure consistent, precise membrane performance from lot to lot and blot to blot. Our membranes set industry standards for reproducible results, durability, and high signal-to-noise ratios. Choose from a range of commonly used formats including rolls, discs, sheets, or custom cuts.

Chromatography

Many tools and techniques are available today for protein purification. Development of a purification process requires an analysis of various strategies to isolate a protein of interest. Protein purification from a complex sample, such as cell culture or serum, requires more than one chromatographic step.
Many tools and techniques are available today for protein purification. Development of a purification process requires an analysis of various strategies to isolate a protein of interest. Protein purification from a complex sample, such as cell culture or serum, requires more than one chromatographic step.
Read more

Western Blotting

Western transfers are an important tool for proteomics research, diagnostics, and forensics applications. This method is widely used to detect the presence of proteins in unknown or complex sample mixtures as well as determining the relative abundance of specific proteins.
Western transfers are an important tool for proteomics research, diagnostics, and forensics applications. This method is widely used to detect the presence of proteins in unknown or complex sample mixtures as well as determining the relative abundance of specific proteins.
Read more

Protein concentration and buffer exchange

Biomolecule purification involves a complex series of steps where targets are selectively separated through sequential processes. Pall offers several technologies to perform efficient sample concentration, desalting, and buffer exchange including ultrafiltration (UF) spin filters, UF multi-well filtration plates.
Biomolecule purification involves a complex series of steps where targets are selectively separated through sequential processes. Pall offers several technologies to perform efficient sample concentration, desalting, and buffer exchange including ultrafiltration (UF) spin filters, UF multi-well filtration plates.
Read more

Protein fractionation

Fractionation using ultrafiltration of complex protein solutions and separation of unbound or unincorporated label from protein samples are some of the main applications of these centrifugal devices.
Fractionation using ultrafiltration of complex protein solutions and separation of unbound or unincorporated label from protein samples are some of the main applications of these centrifugal devices.
Read more